Paper Title
In Vitro Antiprotozoal Activity of Phenolics And Entadamide A Isolated from Entadarheediiagainst Sleeping Sickness, Chagasdisease, and Leishmaniasis

Abstract
Entadarheedii (Fabaceae) seedswere tested for itsin vitro antiprotozoal activity against Trypanosoma bruceibrucei, T. b. rhodesiense, T. cruzi, and Leishmaniainfantum as well as toxicity against MRC-5fibroblast cells.Thetotal crude ethanol extract together with the previously isolated phenolics (protocatechuic acid C1, protocatechuic acid methyl ester C2, andphaseoloidinC3) and a thioamideentadamide A C4 were screened in this study. The ethanol extract showed no activity (score 1) against all tested protozoa.On the other hand, moderate activity (score 3) was exhibited by phaseoloidinagainst all tested protozoa (T. b.brucei IC509.70 μg/mL, T. b. rhodesiense 7.83 μg/mL,T. cruzi 8.00 μg/mL, and L.infantum 6.96 μg/mL). Entadamide A exhibited moderate activity (score 3) against (T. cruzi IC50 8.98 μg/mL and L. inflata IC50 10.77μg/mL). Protocatecheuic acid showed moderate activity (score 3) against T. bruceibrucei,(IC508.12μg/mL) while protocatechuic acid methyl ester showed no activity. All the active tested compounds have low toxicity against MRC-5 cell line (CC5013-37μg/mL).The best selectivity index (SI) was presented by phaseoloidin(4.01 T. b. brucei, 3.93 T. b. rhodesiense, and 4.51 L. infantum). Entadamide A showed the best SI (3.82) towardsT. cruzi. The biologically active antiprotozoal compounds of E. rheediiacting in their pure form rather than acting collectively in total extract.