Paper Title
Encapsulation of Active Fraction from Gymnema Sylvestre and its Effect on Invitro Antidiabetic Activity

Abstract
Microencapsulation of active ingredient from Gymnema sylvestre(TG) was achieved by spray drying technique using maltodextrin (MD) as carrier material. The cross-linking of the active biomolecules were confirmed using FTIR analysis.Further characterization of the microformulation was done using particle size analyzer and Scanning Electron Microscopy (SEM). The percentage efficiency of the microencapsulated TG was calculated as 70±0.14%. The antidiabetic effects of the microformulationwere investigatedinvitro.It showed effective inhibition against yeast α-glucosidase and pancreatic α-amylase with IC50 values 30.48 ± 0.39 and 8.37 ± 0.60 µg/mLrespectively. The microformulation retained the activity at acidic pH (2-3.5) and exhibited significant temperature stability compared to the non- encapsulated active fraction. Further, MTT assay was performed to evaluate the cytotoxic effects of the microformulation. The encapsulated form did not exhibit any toxicity upto 650 µg/mL. The microformulationwas evaluated for its antidiabetic activity by performing glucose uptake assay in 3T3L1 preadipocytes. The extract loaded with the encapsulated material showed significant antidiabetic effects (p≤0.5) when compared to the non-encapsulated active fraction from Gymnema sylvestre. Thus, the microencapsulated active ingredient from Gymnema sylvestre could be considered as a safe and potential nutraceutical candidate for diabetes management. Keywords - Diabetes, Gymnema sylvestre, Microencapsulation, spray drying, GLUT 4, glucose uptake