Paper Title
Pre-Osteoblastic Cells Proliferative Property Of Bioactive Compounds In Germinated Brown Rice: A Promising In Vitro Potentials In Osteoporosis

Abstract
Background: Application of brown rice in the area of biomedical research is increasing especially in the management of chronic metabolic diseases, butadequate effort has not been made to study the cellular toxicity and proliferative ability of its bioactives to properly ascertain its safety. Method: In this study, different in vitro assays were carried out to determine cell proliferation using Trypan blue exclusion assay (TBE) and Bromidine uracil assay(Brdu). Toxicity of the bioactives was measured by (MTT) and Neutral red assays (N.R) on MC3T3-E1 osteoblastic cells at varying concentrations. Alkaline phosphatase activity(ALP), collagen and protein synthesis of the cells were also quantified. Results: A time-dependent, significant proliferative effect on the cells was observed at a concentration of 10g/ml compared to other concentrations of 100 and 1000g/ml for TBE and Brdu (p<0.05). No toxicity was recorded at a concentration between 0 and 1000g/ml, using both MTT and N.R assays. An overall significant increase in alkaline phosphatase activity (p=0.0194) was observed. Collagen and protein synthesis increases significantly in ASG treated cells (p<0.05). Significant correlation was observed between Brdu and TBE results (r2= 0.842, p= 0.01). Conclusions: GBR-bioactives stimulates bone cell proliferation in-vitro possibly by increasing ALP synthesis which leads to new DNA formation, protein and collagen synthesis, and the bioactives has no toxic effect; further research is needed to ascertain the possibility of using these compounds in the management of osteoporosis. Keywords: Cellular toxicity, cell proliferation, GBR- Bioactives, Osteoporosis