Paper Title
Development of Bio-Friendly Drug Delivery Carrier using Surface-Functionalized Chitosan Microsphere

Abstract
Chitosan (CHN) is a polysaccharide consisting of D-glucosamine and N-acetyl-D-glucosamine linked by -(1→4) glyosidic bond. The chemical structure of CHN is shown in Fig. 1. CHN is derived from deacetylation of chitin. CHN has been widely used as biomaterials due to its antibacterial, biocompatible, and biodegradable properties. In particular, CHN molecules are highly positive charged. In this study, we prepared CHN microsphere and investigated its feasibility for a tissue filling and regenerating material. The physical properties of CHN microsphere including size, surface morphology, zeta potential of CHN microsphere and were analyzed. The chemical state of CHN microsphere was also performed using Fourier transform infrared spectroscopy. The interaction of CHN microsphere and proteins was evaluated using positively and negatively charged proteins, i.e. bovine serum albumin and hen egg white lysozyme. The effects of CHN microsphere on proliferation and differentiation of osteoblast-like MG63 cells were evaluated. Our result showed that the submicrosized CHN microsphere was successfully formed and demonstrated its biocompatibility via improving proliferation and differentiation of MG63 cells. Therefore, the CHN microsphere prepared in this study can be potentially applied in the preparation of bone. Acknowledgement - This research was supported by the Ministry of Trade, Industry and energy (MOTIE), Korea, through the education program for Creative and Industrial convergence. (Grant Number N0000717). Index terms - Microsphere, chitosan, drug release, osteoblast.