Fabrication of Biodegradable PCL Microsphere and its Bioactivity on Osteoblast
Advances in biotechnology greatly increased the expansion of human life expectation. Accordingly, the frequency of the age-related diseases become a serious problem. Bone-related disease, i.e. osteoporosis, become a serious problems that deteriorate the quality of life. In this regard, it is strongly desired to implement better resolutions for this problem. Biodegradable polymers have been widely used as biomaterials, bone substitutes in particular. In this study, we fabricated the catechin-incorporated microspheres using a poly(-caprolactone) and examined the physical and chemical properties using various analytical techniques. The size of the prepared microsphere was 40 m in diameter and the surface charge dependent on solution pH was determined using zeta sizer. Drug release kinetics was analyzed using a high-performance liquid chromatography. Cell assays were performed for the evaluation of osteoblastic responses including proliferation and differentiation. Our result demonstrated that the drug incorporation improved the osteoblastic responses and this technique can be further utilized in the preparation of bone substitutes.
Acknowledgement - This research was supported by the Ministry of Trade, Industry and energy (MOTIE), Korea, through the education program for Creative and Industrial convergence. (Grant Number N0000717).
Index terms - Microsphere, PCL, biodegradability, osteoblast.