Paper Title
Antimicrobial Activity of Pitsecellobium Dulce (ROXB) Benth. Root Extract

Abstract
The present study was to evaluate the antimicrobial activity of Pithecellobium dulce root against five microorganisms by using Agar well diffusion and broth microdilution methods. The roots of P. dulce were continuously macerated with ethanol and water respectively. The results showed that the ethanolic extract active against Staphylococcus aureus and Bacillus subtilis that showed the largest inhibition zone of 11.67±0.58 mm against Bacillus subtilis, the lowest MIC of 250 μg/ml against Staphylococcus aureus and the lowest MBC of 500 μg/ml against Bacillus subtilis. The water extract did not showed the activity against Escherichia coli and Saccharomyces cerevisiae but showed the largest inhibition zone of 8.00±0.00 mm and the lowest MIC of 62.5 μg/ml against Staphylococcus aureus, the lowest MBC and MFC of >2000 μg/ml against Staphylococcus aureus, Bacillus subtilis and Candida albicans. The results of the present study refer that P. dulce extracts possess bioactive compounds having important antimicrobial properties. Therefore, the plant extracts can be subjected to further isolation of therapeutic antimicrobial agents for pharmacological and toxicological evaluation. Keywords- Pitsecellobium dulce, Antibacterial activity, Antifungal activity