Epigenetic Patterns in Acute and Chronic Leukemia Cell Line
Leukemia is a group of cancers that usually begin in the bone marrow and result in high numbers of abnormal while blood cells. To unravel the distinctive epigenetic patterns in various leukemia subtypes, two cell lines representing two leukemia subtypes ALL (Acute myelogenous leukemia) and CML (Chronic myelogenous leukemia) were chosen for comparison. Peripheral blood mononuclear cell (PBMC) from normal healthy donors was used as control. PBMC was derived from peripheral blood cell with a round nucleus, which consist of lymphocytes (T cells, B cells, NK cells) and monocytes. Comparative analysis of epigenetic alterations between the AML and CML subtypes of leukemia, particularly in histone modifications and DNA methylation, may provide clue to reveal widespread distinct patterns of epigenetic alterations in leukemia and thereby gain important insight into tumorigenesis.
Keywords - Leukemia, epigenetic regulation, DNA-methylation.