Antimicrobial Activities Of Chitosan Base Nanomaterials
Chitosan can be obtained by deacetylation of chitin, second most abundant organic compound, after cellulose. Chitosan is a polymer made of β-(1→4)-linked D-glucosamine, still having various percentage of N-acetyl-D-glucosamine (acetylated unit). Due to its chemical structure, chitosan has a large number of commercial and biomedical applications. Many of the applications are based on antimicrobial properties of chitosan, properties due to the fact that the polymer contains positive charges. In its free polymer form, the antimicrobial activity of chitosan depends on its concentration, molecular weight and degree of substitution. The development of micro- and nanoparticles of chitosan and its derivatives can conduct to nanomaterials that may have different properties comparing with those of soluble form of the polymer. The chitosan nanoparticles may possess modified physical and morphological characteristics that will modify the initial properties and consequently the initial applications of soluble free form of polymer. In this study, the antimicrobial properties against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicansof soluble free form of chitosan and nanoparticles chitosan based materials, obtained by coacervation / precipitation method and by ionotropic gelation method, were compared. The results revealed that the methods of preparation of chitosan nanoparticles are responsible for the differences in the antimicrobial activities of tested chitosan based materials.
Acknowledgements:This work is supported by GRANT: 14/2017, PNIII-P3-285 GoNanoBioMat - Polymeric nanobiomaterials for drug delivery: developing and implementation of safe-by-design concept enabling safe healthcare solutions.