Liquid Biphasic Flotation (LBF) System: Integration Of Fermentation And Recovery Process Of Lipase From Burkholderia Cepacia
Conventional trend of biomolecules production involves the upstream fermentation process and the downstream comprising separation and purification processes. The extensive market growth of industrial biotechnology over the past decade has created the demand for the development of novel and innovative upstream and downstream techniques for proteins and enzymes. Integrating the upstream and downstream processes in a single system appears to have immense potential in enhancing the efficiency of many bioprocesses. Liquid Biphasic Flotation (LBF) is a new recovery method that has been successfully used as alternative for liquid-liquid extraction process of biomolecules. This method is based on the incorporation of the principles of Aqueous Two Phase System (ATPS) and Solvent sublation (SS). The aim of this research is to integrate the fermentation process of lipase from Burkholderiacepacia and subsequently extract lipase from the fermentation broth via LBF system. From the findings, it has been demonstrated fermentation using LBF system enables the bacteria to grow faster and produce higher yield of lipase compared to the conventional shaker method. Subsequent lipase separation from the fermentation broth was successfully extracted with efficiency reaching 82.67% and with lipase yield of 80.67%. The combination of upstream and downstream processes in a single system enables the acceleration of product formation, improve the product yield, and facilitate downstream processing. In addition, high separation efficiency, simple operation and low environmental impact can be attained from this single system unit. This integration LBF system is potentially attractive application for biomolecules fermentation and separation that possibly open new opportunities for industrial production.
Index Terms: Upstream; Downstream; Bioprocess;Liquid biphasic flotation (LBF); Lipase