Antigenotoxic Potential of Tanacetum Parthenium Leaf Extract and Synthetic Compound Parthenolide against Radiation Induced Micronuclei Formation and Oxidative Stress in Swiss Albino Mice
Background: Radiotherapy is frequently used as a part of cancer treatment to achieve tumor control. However, treatment has been widely used as an effective tool to kill tumor cells; it might produce harmful effects to surrounding healthy tissues. Radiations induce oxidative stress on target tissues, mainly through the generation of reactive oxygen species (ROS) resulting in imbalance of the pro and antioxidants in the cells, in DNA, lipids, and proteins, eventually inducing cell death. Literature review reveals that Tanacetum species have been used as remedies in traditional medicine since ancient times throughout the world.
Objective: To evaluate the antigenotoxic potential of Aqueous and Ethanolic extracts of Tanacetum parthenium (Linn.) and synthetic compound Parthenolide against Electron beam radiation (EBR) induced micronuclei formation and oxidative stress in Swiss Albino mice.
Methodology: Radiation induced micronuclei was performed in the bone marrow of mice according to the method of Hosseinimehret al., 2003. The oxidative stress was carried out to estimate Lipid peroxidation markers (MDA) in the liver homogenate of the mice according to the method of Subash et al., 2011. The experimental animals were orally administered with 100mg/kg b.wt. ethanolic and aqueous extracts of Tanacetum parthenium and 4mg/kg b.wt. Synthetic compound parthenolide once daily for 15 days before exposure to sub-lethal dose (6Gy) of Electron Beam Radiation. Control (C), Radiation Control (RC) and drug control (DC) group was maintained.
Results: The number of Micronucleated Polychromatic Erythrocytes (MnPCE) and Micronucleated Normochromatic Erythrocytes (MnNCE) were decreased in pre treatment groups when compared to radiation control group. Pre treatment was able to reduce Malondialdehyde (MDA) level when compared to radiation control group.
Conclusion: Present findings reveal the antigenotoxic potential of micronuclei formation and reduced lipid peroxidation which may be attributed to scavenging of radiation induced free radicals.
Keywords - Tanacetum parthenium, Electron Beam Radiation, Swiss albino mice, Malondialdehyde and Micronucleus