Paper Title
Swcnts-Based Biosensor For The Detection of Peanut Allergen-Induced Protein

Abstract
We developed a biosensor for detection of Ara h1, one of serious peanut allergen molecules in food. The developed biosensor utilized target of antigen-antibody reaction. Single walled carbon nanotubes (SWCNTs) had a role of transferring the reaction of a biomolecule in a measurable signal to electric response and the optimum concentration assembled on the biosensor platform was assessed. 1-pyrenebutanoic acid, succinimidyl ester (1-PBSE) was used as a linker linking SWCNTs and antibodies and the optimized concentration of 1-PBSE absorbed onto SWCNTs was determined with 1gm/L. Each material was bound as a series of sequence. The electric resistance response was measured by the potentiostat. Also, we confirmed biosensor capacities such as limit of detection (LOD), detection range, washing times and specificity. The biosensor can detect 1 ng/mL of Ara h1 as LOD, the detection range was 1-1000 ng/mL, and the optimal sensing surface washing with deionized water was considered as three times. Indirect enzyme-linked immunosorbent assay (ELISA) was successfully applied to confirm the specificity of antibodies using 96-well microplates.