Organosulfur Compounds of Allium Tuncelianum (Kollmann) Ozhatay, B.Mathew & Şiraneci Extracts By Spme/Gc-Ms And Determining Their Cytotoxic Effect on Hela Cells
Allium tuncelianum (Kollmann) Özhatay, B.Mathew & Şiraneci, known as Tunceli garlic, is an endemic species native to Turkey and distributed only in and around Tunceli district. Tunceli garlic was identified as a subspecies of A. macrochaetum Boiss. et Hausskn., however, following studies identified it as a different species, and it was named as A. tuncelianum. Because its chemical structure is similar to cultivated garlic (A. sativum), it is collected from the mountains, and sold/ used as a commercial product with the name “Rock Garlic” by local people. The plant is important in terms of being endemic and placed in the group of Vulnerable Plants in Red Data Book of Turkish Plants. In the present study, organosulfur compounds, categorized in volatile fatty acids, of the plant, Allium tuncelianum were extracted with solid phase micro extraction (SPME/PDMS) method and analyzed with Gas Chromatography-Mass Spectroscopy device. As a result of the analysis, diallyl disulphide compound was determined to be major compound at the rate of %50.51. Later on, cytotoxic activity of plant extracts was determined on HeLa cells originating from human cervical carcinoma and it was found that 72 h, 0,5 mg/ml concentration led 30 percent of the cells to apoptosis. It is clearly advantageous to rehabilitate Allium tuncelianum plant by cultivating it, increase its production, and making the plant consumed on a global basis rather than its limited consumption by local people, as an alternative to Allium sativum (cultivated garlic) which has been consumed traditionally over centuries.
Keywords- Allium tuncelianum, Solid Phase Micro Extraction (SPME), GC-MS, Cytotoxic Activity, HeLa Cells