In Vitro Antimicrobial Activity of Root Extract of Clitoria Ternatea
Introduction and Objectives:
Infections caused by gram negative bacteria are important causes of morbidity and mortality and have a major impact on public health. Extracts of plants and herbs like Clitoreaternatea (C.ternatea) are used as diuretic.This work attempts to find out antimicrobial activity of aqueous and alcoholic extract of C. ternatea roots against Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC25922), clinical strains of Klebsiella pneumoniae, Escherichia coli and Candida albicans.
The agar well diffusion method was done using Muller Hinton agar and Sabouraud’s dextrose agar. The culture medium was inoculated with the microorganism separately grown in Peptone water. A 4 mm diameter wells punched into the agar was filled with 20μl of aqueous and alcoholic rootextracts C. ternatea extracts in various concentrations (100 μg /mL - 25μg/mL). The plates were then incubated at 37°C for 18 h. The antimicrobial activity was evaluated by measuring the diameter of zone of inhibition. Positive controls were ampicillin10 μg/disc and ketoconazole 15 μg/disc.
The aqueous root extract of C. ternatea with the concentration of 100 μg /ml showed zone of inhibition against Escherichia coli (ATCC 27853) 18mm, Pseudomonas aeruginosa (ATCC 27853) 14mm, multidrug resistant strain of Klebsiella pneumoniae 15 mm.
Alcoholic extract of C.ternatea with the concentration of 100 μg /ml showed zone of inhibition of 35 mm against Escherichia coli (ATCC 27853), Pseudomonas aeruginosa (ATCC 27853) 22 mm, multidrug resistant strain of Klebsiella pneumoniae 28 mm. Candida albicanswas resistant to both aqueous and alcoholic extract of C.ternatea root.
Alcoholic extract of C.ternatea is a better antibacterial agent than aqueous root extractagainst multidrug resistant Klebsiella species and other gram negative pathogens. Further studies are required to identify active substances from the alcoholic extracts of C. ternatea for treating infections.
Keywords— Clitorea Ternatea, Antimicrobial Sensitivity, Multidrug Resistant.