Oxidative stress: a susceptible factor for dna damage in type 2 diabetes mellitus patients in population of west bengal.
Type 2 Diabetes mellitus (T2DM) is a chronic progressive disease affecting a significant proportion of the population and becoming an epidemic worldwide. Increasing evidence in both experimental and clinical studies suggest oxidative stress (OS) plays a major role in the pathogenesis of both types of diabetes mellitus and its complications. OS leads to damage of cellular biomolecules such as lipids, proteins and DNA. OS enhance the extent of DNA damage. Our objective is to detect the extent of DNA damage and to investigate the relationship between DNA damage and oxidative stress in T2DM patients and healthy controls.A case-control study of forty seven patients with T2DM (n=47) and sixteen healthy control subjects (n=16) were screened from different areas of West Bengal. All procedures were done with the informed consent of participants. Comet assay was used to detect DNA damage. Hence, in the present study, increased DNA damage was observed in T2DM patients than healthy controls that may be due to increased oxidative stress leading to genomic instability, which in turn may lead to the progression of T2DM and its complications and contribute to an increased risk for cancer. Therefore this study will explore the role of oxidative stress for genomic instability and causing cancer.
Keywords- Oxidative stress, DNA damage, comet assay, genomic instability.