Effect of Interaction Between Different Concentratons of Oryzalin and Shaking at Different Time Duration on Chromosome Polyploidy of Crepis Capillaris Without B Chromosme Via Tissue Culture Technique.
These experiments were conducted at Tissue Culture Laboratory/ Faculty of Agriculture and Forestry/ University of Duhok during the period from January 2011 to May 2013. The goal of this study was to study the influence of interaction between shaking different concentrations of oryzalin 0.0, 35, 45 and 55 Ám (five and ten days) on chromosome number of Crepis capillaris (without Bchromosomes) through long term callus culture until regeneration of plants from the callus. The result illustrated from tables (1and 2) the interplay between four oryzalin concentrations and two time durations manifest that the treatment of Crepis capillaris calli without B chromosome with 55 ÁM oryzalin on ten days shaking registered a high percentage of polyploidy; however, the callus remain in diploidy stage during the passage 7 until passage 10.
Index Terms- Crepis capillaris, chromosome, B chromosome, tissue culture.