Paper Title
Optimization Of NANO Noisome Drug Delivery System

Abstract
Non-ionic surfactant based vesicle called “Noisome” are a class of vesicular nano carriers. Noisome and liposomes are extensively used in drug delivery systems. Noisome are self-assembly of non-ionic surfactants whereas liposomes are formed from phospholipids. Noisome do not have problems of phospholipid oxidation, hydration in liposome and are biodegradable, biocompatible, nontoxic and capable of encapsulating large quantities of pharmaceutical in a relatively small volume of vesicles. A noisomal structure has been developed that could encapsulate pharmaceutical reagents as a drug delivery system. It has been tried to optimize the fabrication process by experiments which were designed based on central composite design (CCD) to evaluate the effect of four variable parameters including: molar ratio of surfactant, molar ratio of cholesterol, hydration temperature and sonication time. The vesicles were prepared by film hydration method using different mole ratios of surfactant and cholesterol as vesicle forming agents. The influence of formulations on vesicle size, entrapment efficiency and stability of noisomes was investigated. Dynamic light scattering (DLS) was employed to investigate the size of prepared noisomes. The smallest vesicle average sizes were 44 nm and 56 nm without and with cholesterol, respectively. The percentages of entrapment efficiency (%EE) of DETA-NONOate as the drug encapsulated noisomes varied between %66 and %97.6. Optimum condition with the most stable and highest entrapped formulation was 1:1:1/2 for Span60:Tween60 and cholesterol ratios. Moreover, in vitro release of drug from noisomes was investigated in phosphate buffer saline (PBS) with pH=7.4 at T=37 °C. Keywords: noisome, nano, drug delivery, angiogenesis, release, optimization.