16s Rrna-Based Identification Of Atrazine-Degrading Bacteria From Soil Of Thailand
Fourteen Thai degrading-bacteria were isolated from sugarcane field soils in central and eastern of Thailand. All these fourteen isolates were gram-negative with short rod shape. The partial 16S rDNA genes were amplified by polymerase chain reaction (PCR) using the universal primers of 16S rDNA gene. In this study, phylogenetic tree was constructed using a partial sequence within the 16S rDNA gene (approximately 1,200 bases). Strains were grouped into four phylogenetic branches with some subclusters. Based on sequence comparisons and phylogenetic tree analyses, the bacterial isolate studied were identified as a new strains of genus Pseudomonas, Burkholderia, Klebsiella, Enterobacter, Kosakonia and Rhizobium.
Index Termsó 16S rRNA, Atrazine-Degrading Bacteria, DNA sequencing, Identification, Phylogenetic analysis