Paper Title
Qualitative and Quantitative Phytochemical Analysis and Antioxidant Potential of Amaranthus viridis L. From Pakistan

Abstract
Purpose of this study was to conduct qualitative and quantitative phytochemical analysis of Amaranthus viridis and to assess its antioxidant activity by using different assays. Ethnolic extract was prepared from A. viridis leaf powder and its qualitative phytochemical screening was done. Then quantitative phytochemical screening was carried out for the detected phytochemicals. Antioxidant activity analysis was carried out by using DPPH Radical Scavenging Activity assay, ABTS Radical Cation Assay, Phosphomolybdenum Assay and Hydrogen Peroxide Scavenging Activity assay. Qualitative Phytochemical screening resulted in the presence of alkaloids, saponins, flavonoids, tannins, total phenolics and absence of terpinoids and resins in A. viridis leaf extract. Quantitative phytochemical screening showed the amount of alkaloids was 93mg/g followed by flavonoids (8mg/g), saponins (4.4mg/g) and tannins (2.7mg/g). TP contents of A. viridis were 490mg/100g. All the four assays used for radical scavenging activity showed the presence of high oxidative potential of A. viridis. IC50 values were in this order; 12.60�g/ml (Phosphomolybdenum assay), 13.22 �g/ml (ABTS assay), 27.97 �g/ml (DPPH assay), 52.77 �g/ml (Hydrogen peroxide assay). Results showed the high antioxidant activity of A. viridis which is due to presence of high phenolic contents revealed by phytochemical analysis. Index Terms- Phytochemical; Antioxidant; Amaranthus Viridis; Scavenging Activity; Radical