Paper Title
ANTI-ADIPOGENIC EFFECTS OF PROBIOTICS ON LIPID ACCUMULATION IN 3T3-L1 ADIPOCYTES: POTENTIAL FOR OBESITY MANAGEMENT

Abstract
Obesity constitutes a significant global health challenge that is correlated with a plenty of metabolic disorders, stemming from an imbalance between caloric intake and expenditure, which ultimately results in an excess of adipose tissue. Recent research posits that gut microbiota may play a pivotal role in the modulation of obesity through its impact on lipid metabolism. This investigation examined the anti-adipogenic properties of four distinct probiotic strains—Bifidobacterium animalis TISTR 2591 (BA-2591), Enterococcus faecium TISTR 2954 (EF-2954), Enterococcus faecium TISTR 2605 (EF-2605), and Bacillus coagulans TISTR 2853 (BC-2853)—concerning lipid accumulation within 3T3-L1 adipocyte cells. The assessment of cytotoxicity indicated that all investigated strains exhibited non-toxic characteristics at concentrations spanning from 0 to 400 μg/mL. Evaluations of lipid accumulation disclosed that BA-2591 and BC-2853 significantly diminished lipid accumulation in a dose-dependent fashion, whereas EF-2954 and EF-2605 demonstrated no significant effect. These findings imply that specific probiotic strains may exert an influence on adipogenesis, with BA-2591 and BC-2853 exhibiting potential anti-adipogenic properties. Although the 3T3-L1 cell model offers valuable preliminary insights, additional in vivo investigations are essential to corroborate these findings and elucidate the underlying molecular mechanisms. This research underscores the prospective role of probiotics in the management of obesity through the modulation of adipocyte lipid accumulation, thereby presenting a promising avenue for the development of probiotic-based therapeutic strategies. Keywords - Probiotics, Adipogenesis, Lipid Accumulation, Obesity Management, 3T3-L1 Adipocytes.