Paper Title
SCREENING OF PLASMA EV BIOMARKERS BASED ON AFFINITY MAGNETIC BEADS

Abstract
Plasma-derived extracellular vesicles (EVs) are promising sources of biomarkers. Currently, it is still a challenge to isolate EV from a small amount of human plasma for downstream proteomic analysis. The separation process is hindered by contamination with high-abundance blood proteins and lipoprotein particles, which negatively affect proteomic analyses.To address these issues, we have developed a functional affinity magnetic bead, EVlent（Extracellular Vesicles isolated Efficiently, Naturally, and Totally）, enabling the rapid and efficient separation of EVs from plasma.By optimizing the quantities of magnetic beads and plasma used, we characterized the isolated EVs through WB, NTA, and transmission electron microscopy (TEM). The results showed that we successfully separated EV from plasma. Subsequent proteomic analysis of these EVs identified over 2000 proteins and 15000 peptides from using just 100μL of plasma. Additionally, compared with UC, this separation method significantly reduces pollutants, including plasma proteins and lipoproteins.Finally, we applied this strategy to plasma samples of healthy people and Parkinson's disease, and screened out potential biomarkers, which provided a promising guidance for clinical diagnosis. Keywords - Plasma; Extracellular Vesicles; Affinity Magnetic Beads; Parkinson's Disease.