Paper Title
ONE-POT EXTRACTION TECHNIQUE FOR SIMULTANEOUS ANALYSIS OF EV PROTEIN AND miRNA

Abstract
The cargo of extracellular vesicles (EVs), encompassing proteins, nucleotides, and various other molecules, has emerged as a powerful tool for the discovery of potential disease biomarkers, crucial for diagnosis, monitoring, and therapeutic intervention. However, the intricate relationship among these cargos remains enigmatic, potentially due to the limitations of current EV cargo extraction methods, which often target a single cargo type.To address this challenge and enhance our analytical capabilities with regards to EV cargo, we introduce a groundbreaking one-step pipeline that facilitates the concurrent extraction of EV proteins and miRNAs. This innovative strategy seamlessly integrates the advanced EVtrap separation technique with solid-phase-enhanced sample preparation (SP3), enabling a comprehensive analysis of urinary EV proteins and miRNAs.In comparison to traditional methods, DIA MS and NGS results are displayed that our technology boasts superior extraction efficiency and purity, while significantly reducing operational complexities and time constraints. When applied to the detection of prostate cancer protein and miRNA markers, our technique yielded impressive results, demonstrating its precision in capturing key molecules and providing robust support for early prostate cancer diagnosis.This study not only marks a significant technological advancement in the concurrent extraction of EV proteins and miRNAs, but also serves as a valuable resource for tumor marker research and clinical applications. With the widespread adoption and promotion of this technology, we anticipate significant strides in cancer diagnosis. Ultimately, this innovation will pave the way for more precise and timely detection and treatment programs, ultimately leading to improved patient outcomes. Keywords: Extracellular vesicles, Protein, MiRNA, Prostate cancer, Biomarker.