Paper Title
GARCINOL, A NOVEL ANTITHROMBOTIC AGENT: INHIBITION OF PLATELET ACTIVATION AND INTEGRIN αIIBβ3-MEDIATED SIGNALING PATHWAYS

Abstract
Platelets hold a central role in both normal hemostasis and pathological arterial thrombosis, activated by adhesive proteins or soluble agonists that induce signal transduction through their respective receptors. This activation leads to common signaling events, triggering platelet shape changes, inside-out signaling, and fibrinogen binding to integrin αIIbβ3, initiating outside-in signaling, platelet granule secretion, and aggregation. Extracted from the dried fruit rind of Garcinia indica, garcinol, a polyisoprenylated benzophenone, exhibits diverse bioactivities. Despite limited research on its impact on platelet activation, this study reveals garcinol's inhibition of aggregation induced by collagen, thrombin, arachidonic acid, and U46619.Garcinol directly inhibited integrin αIIbβ3 activation by interfering with FITC–PAC-1 binding stimulated by collagen. In addition, garcinol affected integrin αIIbβ3-mediated outside–in signaling. In conclusion, this study identified that garcinol, a novel antithrombotic agent, acts as a naturally occurring integrin αIIbβ3 inhibitor. Keywords - Antithrombosis, Garcinol, Integrin αIIbβ3, Human Platelet