Paper Title
INFLAMMATION CORE FACILITY IMPROVES PATHOLOGICAL RESEARCH IN PSORIASIS, A TYPE OF INFLAMMATION-RELATED DISEASES

Abstract
Inflammation is relevant to a large number of immune responses and results in many diseases. Our research group is the inflammation core facility (ICF) and focuseson developingthe study of inflammation-mediated diseases.Our objective is to provide multi-plex immunoassay (MPI) to measure mediator levels in samples. The other objective is to analyze the infiltration of immune cells in certain tissues through hematoxylin plus eosin (H&E) and immunohistochemistry staining (IHC). ICF also provides animal mouse model service including model development and the subsequent analysis by MPI and IHC. We have testedpsoriasis by injection intradermally of IL-23 in mice to compare the function of threedrugs in the regulation of psoriasis by MPI to determine cytokine levels, and byH&E and IHC to study the cells contributed to psoriasis. The results indicate that three drugs can reduce the ear thickness of mice. The H&E also showed a similar function of the two drugs. The MPI results show no difference among the three drugs. We have performed immunospot (ELISPOT)to analyze the numbers of mediator-secreting cells collected from sera and organelles from animals or human. There are many cytokine-secreting cells from the mouse splenocyte stimulated with Con A, we have detected ~5-1000 cytokine-secreting cells in 100,000 cells by ELISPOT. The function of the drug to the number of cytokine-secreting cells in psoriasis will be tested to confirm the function of the drug in psoriasis. Further study will be applied to evaluate the function of drugs in immune cells and mediators in the regulation of psoriasis. Keywords - Inflammation, Immunoassay, Immunohisto Chemistry Staining, Psoriasis, Cytokine