Paper Title
The Next-Generation Disinfectant: Bacterial Inactivation Efficacy Using Plasma Activated Water Prepared with Two Different Plasma Reactors

Abstract
In this comparative study the bacterial inactivation efficiency of plasma-activated water (PAW) generated by two distinct plasma reactors: one utilizing a nitrogen plasma jet electrode and the other a hybrid argon plasma reactor was explored. Present study involved the assessment of antimicrobial activity against suspension of three Gram-positive and three Gram-negative bacterial strains in their planktonic cell state. Each bacterial suspension was inoculated into PAW five days after generation, and the viable counts at different exposure times of 0.5, 1, 3, 5, 10 and 24 hours were measured to determine the inactivation efficacy. Physicochemical properties of PAW, including pH, conductivity and concentrations of H2O2, NO2¯, NO3¯ during aging were measured. Present study demonstrated the effective inactivation of the tested bacterial strains by PAW. Gram-positive bacteria displayed greater resistance compared to Gram-negative species, with the lowest reductions in bacterial counts observed for B. cereus, and highest for Escherichia coli O157:H7. Physico-chemical measurements showed slow decay of the reactive species in the aging process. This study illustrated the potential utility of PAW as an alternative disinfectant. Keywords - Bacteria inactivation efficiency, Gram-positive bacteria, Gram-negative bacteria,Plasma activated water, Plasma jet, Hybrid plasma discharge