Paper Title
Isolation and Screening of Actinomycetes Producing Antimicrobial Substances Against Pathogenic Microorganisms

Abstract
A total of 6 actinomycete strains were successfully isolated from 4 medicinal plant rhizospheres. The genetic relatedness amongst these isolates was determined based on Random amplified polymorphic DNA (RAPD) and Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC PCR). Both methodologies generated specific patterns corresponding to particular genotypes. ERIC PCR fingerprinting proved to be slightly more discriminatory than RAPD. However, among all isolates tested, the PN11 and PN 12 isolated from rhizosphere soil of Boesenbergia rotunda exhibited the activities against both of pathogenic bacteria and fungi including Bacillus cereus, Staphylococcus aureus, Escherichia coli, Bipolaris sp. and Fusarium sp. According to morphological characterization and analysis of 16S rDNA sequencing of those 2 isolates showed that they belong to genusStreptomyces. Keywords - Streptomyces, rhizosphere soil, Antimicrobial Substances