Paper Title
INHIBITION OF KKU-M213 CHOLANGIOCARCINOMA CELL INVASION BY RHINACANTHIN-C EXTRACTED FROM RHINACANTHUS NASUTUS (L.)

Abstract
Abstract - Cholangiocarcinoma (CCA) is a malignant tumor of bile duct epithelium associated with a slow growing but rapid metastatic and high mortality rate. Rhinacanthin-C, a naphthoquinone isolated from roots of Rhinacanthusnasutus KURZ (family Acanthaceae), has been reported to possess various biological benefits including anti-proliferation activity and induction of apoptosis in several cancer cell lines.Thus, the aim of this study was to test the feasibility of the hypothesis that rhinacanthin-C may inhibit the migration and invasion in KKU-M213 cell line. Rhinacanthin-C significantly inhibited the proliferation in KKU-M213 cells.Rhinacanthin-C significantly inhibited the migration in KKU-M213 cell line in a dose-dependent manner.Using invasion chamber assay, rhinacanthin-C significantly inhibited invasion activity in KKU-M213 cells in a dose-dependent manner.Therefore, rhinacanthin-C is able to inhibit CCA cell migration and invasion in vitro.Therefore,rhinacanthin-C may deserve as the potential agents for the treatment of cholangiocarcinoma metastasis. Keywords - Rhinacanthin-C, Invasion, Cholangiocarcinoma, KKU-M213