Paper Title
HISTOPATHOLOGICAL AND BEHAVIORAL IMPAIRMENTS IN ZEBRAFISH (DANIO RERIO) CHRONICALLY EXPOSED TO A COCKTAIL OF FIPRONIL AND PYRIPROXYFEN

Abstract
Abstract - The aim of the present study was to investigate the impact of Fipronil (FIP) and pyriproxyfen (PYR) mixture on zebrafish tissues and behavior. To assess the influence of this cocktail on zebrafish, three groups of animals were randomly selected and exposed to 0, 0.05 and 0.1 mg L-1 FIP and PYR mixture for 5 days. The fish were evaluated daily by the T-maze tests as locomotor activity and dark-light test and recorded for 4 minutes. The data was quantified using the EthoVision software. Our results indicated significant changes in locomotor activity parameters showing increased levels following exposure to the mixture of FIP and PYR. On the other hand, the mixture also triggered anxiety in the zebrafish, which spent more time in the light area than in the dark. In addition, mixture-induced histological changes were observed in the form of numerous hemosider in deposits found in various zebrafish tissues. Keywords - Pesticides, Fipronil, Pyriproxyfen, Behavior, Histopathological Changes, Zebrafish.