Paper Title
SCIENTIFIC VALIDATION OF ETHNOPHARMACOLOGICAL ANTI-INFLAMMATORY DRUG AMONG THE IRULA TRIBE SETTLED IN ATTAPADI, KERALA, INDIA

Abstract
Abstract - It is evident that the tribal medicine which is used from ancient times shall be od an immense remedy for current lifestyle diseases. In this study, we are analysing and validating the anti-inflammatory drug by the Irula tribal settlement in Attapadi, Kerala, India. As per the information obtained from the tribal peoples the source (millipede) was collected from in and around the Attapdi forest area during January 2021 and extracts were taken for analysis. The anti-inflammatory activity of extracts was evaluated via the 15-lipoxygenase (15-LOX) inhibitory and the nitric oxide (NO) inhibition assays using lipopolysaccharide (LPS)-activated RAW 264.7 murine macrophages. The results indicated that The hexane extract had good lipoxygenase inhibitory activity with IC50 of 4.65 µg/mL, significantly (p < 0.05) higher than that of the positive control quercetin (IC50 = 24.60). The same extract also had good nitric oxide inhibitory activity with 86% NO inhibition and cell viability of 97% at 50 µg/mL. The antioxidant and anti-inflammatory activities therefore resided in both polar and more non-polar fractions which maybe further taken for drug development.