Solid Phase Extraction Of Chloramphenicol With Bulk Mode Synthesized Molecularly Imprinted Polymer
Molecularly imprinted polymer (MIP) for solid phase extraction (SPE) of chloramphenicol (CAP) was synthesized in bulk mode polymerization reaction using CAP as template and methylmetacrylate (MMA) as functional monomer. The structure of synthesized polymers were characterized by fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). Surface area measurements of obtained polymers were done with BET modelling in nitrogen porosimetry studies, 197.8389 m2/g was calculated for MIP while 3.4127 m2/g area was calculated for NIP. Solid phase extractions of analyte compounds for synthesized polymers have been carried out in batch mode. Adsorption isotherms have been constructed and time dependent adsorption capacities Q (mg/g) have been calculated. Q values for synthesized MIP, NIP and commercial supplied polymer SupelMIP were 2.28 mg/g, 0.82 mg/g and 2.50 mg/g respectively. Solid phase extraction was optimized in methanol:ethylacetate (1:1) solution, CAP recoveries in 5-50 mg/l concentration range varied at 90% - 92%.
Keywords� Solid Phase Extraction, Molecularly Imprinted Polymers, Chloramphenicol.