Paper Title
PHYTOCHEMICAL PROFILING, IN-VITROANTHELMINTIC EVALUATIONAND IDENTIFICATION OF POTENTIAL LEAD USING INSILICO STUDIESFROM THE LEAVES OF POLYALTHIA SUBEROSA (ROXB.) THWAITES

Abstract
Abstract - Polyalthia suberosa, a member of the Annonacea family, is a remarkable evergreen shrub with numerous medicinal properties that have been extensively employed in traditional practices.Recognizing the snippets of information available regarding the leaves' standardization, efforts were undertaken to establish phytochemical standards and conduct in-vitro anthelmintic evaluations to validate its therapeutic potential by employing, molecular docking studies to identify lead molecules, providing additional evidence for its pharmacological activity. Preliminary phytochemical screening revealed the existence of alkaloids, sterols, terpenoids, flavonoids, tannins, and saponins in different extracts. Furthermore, HPTLC fingerprinting, FTIR and GC-MS analysis were performed on the methanolic extract to identify the bioactive compounds present.In-vitro anthelmintic activity of the methanol, chloroform, and hexane extracts of Polyalthia suberosaleaves was evaluated at doses of 10, 20, 40, and 80mg/ml on mature earthworm Lumbricus terrestris.Paralyzing and death time were determined, revealing that all three extracts exhibited significant activity against the standard drug albendazole (10mg/ml).For in silico studies, twelve promising phytocompounds were selected from the GC-MS analysis and subjected to screening against the Ascaris suum mitochondrial rhodoquinol-fumarate reductase (MRFR) and tubulin-colchicine targets. Out of these, six compounds and albendazole exhibited good binding affinity (-6.00 to -9.96 kcal/mol) for both targets.Drug likeness and ADMET studies were carried out. These identified phytocompounds hold great potential as lead compounds, displaying significant anthelmintic activity and should be further examined by in-vivo studies for ensuring its therapeutic activity. The outcomes of this study will be valuable in assessing the quality, purity, and safety of the source taxon. Keywords - Polyalthia Suberosa, Standardization, Methanolic Extract, HPTLC, FTIR, GC-MS, In-Vitro,Anthelmintic, In Silico, MRFR, Tubulin-Colchicine, ADMET