Paper Title
DETERMİNİNG THE RELATİONSHİP BETWEEN PTEN IVS4 (-/+) POLYMORPHİSM AND TYPE 2 DİABETES: A HOSPİTAL-BASED CASE-CONTROL STUDY

Abstract
Background/Objectives - Type-2 diabetes is one of the most common metabolic disorders characterized by decreased insulin secretion caused by pancreatic β-celldys function and insulin resistance in targetorgans. Despite increasing knowledge of risk factors and successful prevention programs, the incidence and prevalence of the disease continues to increase globally. Phosphatase and tensin homolog (PTEN) is a potent tumor suppress or gene that an tagonizes the proto-oncogenic phosphatidy linositol 3 kinase (PI3K)/protein kinase B (Akt) signaling path way and governsessential cellularmetabolic processes. PTEN serves as a negativeregulator of the PI3K/PTEN/Aktsignaling pathway, which regulates cellular functions such as cell growth, differentiation, proliferation, and migration. PI3K/PTEN/Aktsignalingcascadesmay also affect glucose up take viatranslocation of GLUT-4. PTEN functions as a potent negative regulator of insulin signaling and is one of the possible candidates associated with susceptibility to the development of Type-2 diabetes. Methods - In light of this information, our study was planned to determine the relationship between the IVS4(-/+) polymorphism of the PTEN gene and the risk of Type-2 Diabetes. In our study; DNA was isolated from blood samples taken from 100 Type-2 Diabetes patients and 100 healthy individuals. The PCR-RFLP method was used for the evaluation of polymorphisms. Results - 43(-/+), 57(-/-) genotypes were determined in diabetic patients for PTEN IVS4(-/+) variant, while 53(-/+), 47(-/-) genotypes were determined in control individuals. Conclusion - When these data were evaluated, no statistically significant difference was found between the diabetic patients and the control group in the genotype distribution of the PTEN IVS4(-/+) variant (p<0.05). Keywords - Type 2 Diabetes, Insulin Resistance, PTEN, Polymorphism