Paper Title
BACTERIAL BIOFILMS GROWTH ON THE MOUTH CAVITY TISSUE SURFACES OF ORAL CANCER

Abstract
Abstract:- Aim: Microflora growth on oral carcinoma surfaces may lead to both local and systemic infections, which may complicate the morbidity of the patient suffering from oral neoplasms. Anticancer therapies impair the local immunity of the oral mucosa further aggravating the proliferation of the microbial biofilm. This study investigated the microbial contents and biofilms present on the surfaces of oral squamous cell carcinomas. Materials and Methods: Biofilm samples were obtained from the central surface of the lesions and contiguous healthy mucosa of the same patients from the Chhattisgarh state of India, before any antibiotic therapy or any tumor treatment. Samples were transferred to selective media for further studies. Samples were also subjected to the SEM study to check the possible colonial pattern of microbial flora. Results: The average number of anaerobic colony forming units (CFU/ml) at the tumor sites (1.8 x 107) was significantly higher than for the healthy (control). Biofilm-like structure of various microbial species (gram-positive and gram-negative bacteria) was found in the tumor sample in SEM analysis. Conclusions: Human oral carcinoma surface biofilms harbor significant numbers of biofilm-forming microbes as compared with the healthy mucosal surface of the same patient. This finding must be considered concerning the known predisposition of such patients to systemic infections, and the unpleasant complications of oral morbidity due to infected lesions. Besides anti-tumor therapies, healthcare professionals might consider direct antimicrobial treatment to reduce patient morbidity. Keywords - Oral Cancer, Morbidity, Bacterial Infection, Bacterial Biofilm.