Paper Title
PRODUCTION OF SURFACE PATTERNED POLY(Ε-CAPROLACTONE) SCAFFOLDS BY ELECTROSPINNING FOR MANIPULATING CELL BEHAVIOR

Abstract
Abstract - In this study,the main purpose was to createfibrous surface patterns of poly(ε-caprolactone)(PCL) by a mask/stencil integrated with electrospinning which is not performed in the literature before.Scaffolds were produced by solvent casting and patterns were created by electrospinning. In order to characterize scaffolds and e-spun fibers; Scanning Electron Microscopy (SEM) analyses, Contact Angle (CA) measurements, thickness measurements, mechanical tests, and Fourier-Transform Infrared Spectroscopy (FTIR) analyses were done. SEM imagesshowed that the electrospun fibers had uniformand bead-free morphology and solvent-cast surfaces were nonporous and smooth. According to CA measurements, electrospun patterns were hydrophobic while solvent-cast surfaces were hydrophilic. In addition, cell culture assays (cell attachment, MTT assay, SEM imaging, and Fluorescence Imaging) were conducted with the L929 cell line (mouse fibroblast cells) for 7 days. As a result of cell culture studies, it was found that electrospun patterns and solvent-cast surfaces had different effects on cell proliferation and differentiation. Consequently, scaffold characterizations and cell culture results revealed that cell proliferation and attachment could be manipulated by surface patterns. In the future, patterns of various shapes with smaller sizes could be created on the scaffold surfaces by this easy method for tissue engineering applications.