Paper Title
ISOLATION AND SCREENING OF LIPASE, PROTEASE, CELLULASE AND AMYLASE PRODUCING BACTERIA FROM OIL CONTAMINATED SOIL

Abstract
Abstract - Some industries or processes such as waste water treatment process, textile industry and detergent making process require multiple enzymes. Among microbes, Bacilli species can produce multiple extracellular enzymes. In this experiment, spore forming bacteria were isolated from oil contaminated soiland theirextracellular enzyme production and enzymatic activity indexes were screened at pH 7 and 37℃ and calculateded. Different pH and temperature were examined for best growth. Morphological characteristic and biochemical tests revealed that all were gram positive. Among seven isolated strains, six strains were aerobic, rod-shaped Bacillus species and the left one which produced only two kinds of enzymes was facultative anaerobic, filamentous Streptomyces. Out of seven isolated strains, three strains (S1, S3 and S7) secreted lipase, protease, cellulase and amylase, three others (P1, P2 and P11) secreted lipase, protease and cellulase and the left (P105) lipase and protease only. Keywords - Bacilli Species, Multi Extracellular Enzyme Producing Bacteria, Oil Contaminated Soil, Spore Forming Bacteria