Paper Title
Determination of the Activity of Enzymes Produced by Intestinal Bacteria in the Presence of Resistant Dextrin

The aim of the study was to determine the activity of enzymes produced by intestinal bacteria in the presence of resistant dextrin. For this purpose, the concentration of β-glucosidase and β-glucuronidase was determined by spectrophotometric method in the post-culture fluid obtained as a result of joint cultivation of intestinal microorganisms of the genera: Lactobacillus, Enterococcus, Bifidobacterium, Clostridium, Escherichia, Prevotella and Bacteroides, which were isolated from the faeces of children. On the basis of the obtained results, it was found that the resistant dextrin caused a decrease in the activity of all tested enzymes. The concentration of enzymes was lower compared to the control samples, where the carbon source was glucose. The activity of the enzymes varied depending on the cultivation time and reached the highest values at 96 or 168 hours of cultivation. Keywords — Faecal Enzymes, Intestinal Bacteria, Resistant Dextrin