Effects Of Collapsin Response Mediator Protein 2 On Glucose And Lipid Metabolism In Adipogenesis
Diabetes mellitus is a metabolic abnormality characterized by impaired insulin efficacy and susceptibility to develop multiple complications. Alterations in cytoskeleton dynamics are implicated in insulin action, glucose transporter type 4 (GLUT4) translocation and lipid droplets trafficking in adipocytes. Function of the major microtubule-structure regulator collapsin response mediator protein 2 (CRMP2) is modulated by the important insulin signaling mediator glycogen synthase kinase-3β; therefore, we hypothesized that CRMP2 was involved in energy homeostasis in adipocyte differentiation (adipogenesis). The aim of this study was to investigate expression profile and effects of CRMP2 on glucose and lipid metabolism in 3T3-L1 adipogenesis in environment with differential glucose concentrations. Our results showed that CRMP2 expression was significantly increased while lipids contents were reduced in cells differentiated under normoglycemic condition. Glucose uptake activity remained unchanged while expression of fatty acid binding protein 4 (FABP4) and GLUT4 were elevated in cells exposed to low glucose. The above results suggest that under physiological glucose environment, the increased CRMP2 expression results in upregulation of FABP4 and GLUT4 and facilitating adipocyte differentiation. Therefore, CRMP2 may participate in diabetic pathogenesis through mediating lipid and glucose metabolism in response to external nutrient condition.