Paper Title
Relationship Between Polymorphism of Fut1 Gene With Diarrhoea in Wild Pig and Hybrid of Wild Boar with Indigenous Breed Pig in Vietnam

Abstract
Industrial pig farming for a long time has eroded the genetic resources of pig breeds. Crossbreeding with wild pigs is considered a method that can restore genetic resources. Alpha-1-fucosyltransferase (FUT1) gene has some reported relative with the disease resistance. The study aimed to survey the relationship between polymorphism in the FUT1 gene and the rate of diarrhoea in wild pigs and hybrid of them with the indigenous pig in Vietnam. For this purpose, five groups of pigs including 61 wild pigs (WP), 51 Mongcai pigs (MC), 32 Soc pigs (SP), 65 F1 hybrid pigs (WPxMC), and 56 F1 hybrid pigs (WPxSP). The FUT1 gene polymorphism in SNP c.307G>A was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods in five groups. The diarrhoea in pigs was observed and followed by pathological features throughout the feeding period. The rate of diarrhoea in pig was 11.48 % (wild pig), 9.80% (Mongcai pig), 21.86% (Soc pig), 13.85% (Hybrid WPxMC), and 12.50% (hybrid WPxSP). The rate of pig dead by diarrhoea was highest in Soc pigs (15.63%) and lowest in wild pigs (4.92%). Results of FUT1 gene polymorphism, both A and G alleles appeared in all studied groups of pigs. All 3 genotypes AA, AG, GG appeared in healthy pigs in 5 studied pig groups. In which the AG genotype has the highest frequency in the Hybrid WPxSP group (58.82%). In the group of pigs with diarrhoea, genotypes AA and GG did not appear in MC and SP group, and the GG genotype did not appear in the group F1(RxS). Diarrhea mortality did not differ between the study groups and there was no correlation between genotypes of SNP site c.307G>A and the rate of pig mortality due to diarrhea. The OR of disease and healthy in allen A/G was height in WP x WP, SP x SP, Hybrid WPxMC, hybrid WPxSP. Keywords: Biodiversity, Crossbreeding, Disease Resistance, Local Breeding, Nucleotide Polymorphism, Swine Genetic.