Neural Stem Cell-Derived Exosome As Endogenous Carrier For Bdnf Delivery To Ischemic Stroke Model
Stroke is the leading cause of death and disability all over the world, with few effective drugs. Currently, the optimal strategies for the treatment of ischemic stroke include stem-cell-based therapy and delivery of drugs, such as functional proteins; However, functional proteins such as BDNF can’t play a therapeutic role in a timely and effective manner due to the harsh microenvironment. We previously demonstrated that human neural stem cell (hNSC)-derived exosomes (hNSC-Exo) alleviate inflammation and improve functional recovery. Here, we have developed a protein delivery strategy based on human neural stem cell exosomes (hNSC-Exo) to improve its therapeutic effect. We load BDNF into exosomes to construct engineered exosomes, and compared their effects with those of hNSC-Exo. The results of our study suggest the potential therapeutic effects of BDNF-hNSC-Exo for stroke. Moreover, the strategy proposed in our study may shed light on the clinical application of other neurotrophic factors for central nervous system diseases.
Keywords - Neural Stem Cell, Exosome, Brain-Derived Neurotrophic Factor, Ischemic Stroke, Regeneration