Paper Title
Timestable Glucose Biosensor Based on Multi-Component Nanoplatform

Abstract
The growing number of diabetic patients became increasingly interested in accurate glucose monitoring.The biorecognition element of enzyme-based amperometric sensors is enzymes, which catalyze substrate conversion. Electrons are transferred from the electrode to the enzyme or vice versa in redox active enzymes to generate a measurable current [1,2].However, one of the more significant problems with these devices is their stability over time. The long-term stability of the biosensor can affect various final properties such as sensitivity, selectivity,repeatability, or the unit cost of measurement [3,4]. Herein, a novel amperometric biosensor based on a multi-component nanoplatform based on magnetite/polydopamine/β-cyclodextrins nanocomposite was fabricated for the detection of glucose. Magnetite nanoparticles were obtained by the co-precipitation method, coated with polydopamine, and β-cyclodextrins were grafted. A process of immobilization of glucose oxidase from Aspergillus niger via adsorption was performed. The resulting platform was used to modify the glassy carbon electrode(GC). To accurately define the obtained systems, versatile physicochemical assays were carried out. Finally, several electrochemical techniques were used to conduct electrochemical tests on various glucose samples. In this work, the operating conditions (pH, temperature, mediator concentration) of the biosensor were optimized. Results for the biosensor showed a broad linear range (1–26 mM glucose), with sensitivity as high as 115.74 μA mM-1 cm-2 and a limit of detection (LOD) of 1.55 μM. The results suggest that the biosensor could be a useful tool in glucose measurement. Furthermore, the proposed biosensor has shown long-term stability(tested up to 9 months), which makes it an attractive alternative to commercial blood glucometers. This work was financed and prepared as part of a research project supported by the National Science Center Poland, no. 2017/27/B/ST8/01506 andsupportedby the Poznan University of Technology research grant no. 0912/SBAD/2118. Keywords - Glucose Measurement, Electrochemical Biosensors, Glucose Oxidase