Nerve Growth Factor- and Retinoic Acid-encapsulated Nanoparticles with Grafted PPFLMLLKGSTR Peptide for Differentiation of Induced Pluripotent Stem Cells
PPFLMLLKGSTR peptide was crosslinked on the surface of solid lipid nanoparticles (SLNs) with entrapped nerve growth factor (NGF) and retinoic acid (RA) (RA-NGF-PPFLMLLKGSTR-SLNs) for guiding differentiation of induced pluripotent stem cells (iPSCs). SLNs prepared in microemulsion displayed appropriate size to entrap NGF and RA. High affinity of surface PPFLMLLKGSTR to iPSC α3β1 integrin facilitated uptake of RA-NGF-PPFLMLLKGSTR-SLNs. Immunochemical staining images showed that the ability of RA-NGF-PPFLMLLKGSTR-SLNs to differentiate iPSCs could considerably increase due to promoted internalization into iPSCs. RA-NGF-PPFLMLLKGSTR-SLNs are potential drug carriers to trigger neuronal production from iPSCs and can be effective in regeneration medicine for neural injury and neurodegenerative disease therapies.
Keywords - Induced Pluripotent Stem Cell, Solid Lipid Nanoparticle, Nerve Growth Factor, Neuron, PPFLMLLKGSTR, Retinoic Acid