Development of Serum Amyloid a Sensing Nanointerface Integrated Polymer Assembly Electrode Detecting Neonatal Sepsis Infections
Neonatal sepsis is a main cause for the increasing rate of morbidity and mortality in neonates across the globe. About 1.6 million deaths in developing countries annually are caused by neonatal sepsis. Neonatal sepsis is a condition caused by bacteria, virus for fungi in bloodstream. Based on the occurrence neonatal sepsis is categorized into two early-onset sepsis (EOS) and late onset-sepsis (LOS). EOS is observed within 72 hrs. of life whereas, LOS is observed from 1 week to 90 days of life caused due to the unhygienic surrounding environment. There are several biomarkers that increase their level during the inflammation. In the presented work an electrochemical biosensor was fabricated based on the imprinting technology for serum amyloid A (SAA) that is a biomarker for neonatal sepsis detection the level of SAA elevates due to the infection within 6-8 hours of occurrence and decreases at normal conditions. For better performance of the biosensor it was modified with different nanomaterials such as multi-walled carbon nanotubes (MWCNTs), manganese oxide nanospheres (MnO2) and cobalt oxide nanoparticles (Co3O4) providing better catalytic property, higher electron conductivity, surface-to-volume ratio. The fabricated biosensor operates in a dynamic detection range 1 µM to 0.01 pM and the detection limit was very low as 0.01 pM.
Keywords - Biosensor; Serum Amyloid A; Neonatal sepsis; Impedance; Nanoparticles