Paper Title
Expression of Proliferative Marker Ki-67 and its Association with Clinic Pathological Parameters in Gallbladder Carcinoma

Purpose: Gallbladder carcinoma is the fifth most common malignancy of the gastrointestinal tract. This study is to evaluate the expression Ki67 in malignant gallbladder lesions, and to establish its correlations with clinicopathologic parameters. Materials and methods: Histopathological analysis was conducted on formalin fixed paraffin embedded malignant gallbladder (n=47) tissue samples. Hematoxylin and eosin stained slides of each case were reviewed for: type of malignancy (whether adenocarcinoma, squamous cell carcinoma, or any other type); grade (well, moderate, and poorly). Immunohisto chemistry for Ki-67 was performed and results are interpreted. Results: The expression of Ki67 in malignant gall bladder was found to be positive in 97 %(n-46) case. In this study expression of >20 % was consider to be positive. Expression ranged from15% to 95 % with average of 53.64. As the grading of disease increased Ki 67 expression also increased. Conclusion: The expression of Ki67 is strongly associated with tumor cell proliferation and growth, and is widely used in routine pathological investigation as a proliferation marker. The present study demonstrated there is significant expression of Ki67 in gallbladder cancer. Greater expression of Ki67 was found with metastatic disease. However less no of early stage disease and a smaller number of patients, requirefuture studieswith a larger number of patients to precisely define the correlation of Ki67 positivity with clinicopathological parameters. Acknowledgment: The present study has been sponsored by Uttarakhand State Council for Science and Technology, Dehradun (UCS&T/R&D/MED-01/17-18/13833) Keywords - Gene Expression, Gall Bladder Carcinoma, Immunohistochemistry,Ki-67