Paper Title
Modified Fermentation Media For Enhanced Production of Surfactin By Local Isolated Bacillus Subtilis

Abstract
The effect of media components that are required in fermentation process to yield microbial product is investigated. In this experiment, the production of surfactin, produced from local isolated Bacillus subtilis 3M was enhanced through modification of Cooper�s media formulation. Three important components selected for enhancement of surfactin yield were glucose, ammonium nitrate, ferrous sulfate and manganese sulfate concentrations. High pressure liquid chromatography (HPLC) was used to determine surfactin concentration contain in the fermentation broth. Modification of glucose, ammonium nitrate, ferrous sulfate and manganese sulfate concentration in Cooper�s media formulation resulted to significant increase in surfactin yield. Application of 30 g/L, 0.05 M, 120 �M and 20 mM of glucose, ammonium nitrate, ferrous sulfate and manganese sulfate, respectively in modified Cooper�s media formulation resulted to 181.1 mg/L of surfactin production compared to 90 mg/L of surfactin using unmodified Cooper�s media. Results obtained in this study provided key parameters for response surface methodology study for further optimization of surfactin using local isolated Bacillus Subtilis. Keywords- OFAT, Optimization, Media Condition, Surfactin