Paper Title
Extraction of Lysozyme from Chicken Egg White by Cross Flow Ultrafiltration using Sulfonated Poly (Ether Sulfone) Membranes

Abstract
Lysozyme is an essential enzyme which kills assured bacteria by breaking u-bonds among the N-acetyl-muramic acid and the N-acetylglucosamine of peptidoglycan on the microbial surface. Currently, lysozyme is broadly applies as food protective and inhibitory beneficial role as an anticancer drug. Therefore, ultrafiltration using sulfonated poly ether sulfone (SPES) cation exchange membranes was developed as simple, effective, and easily scalable methods for separating lysozyme. In this study, synthesis of sulfonated polyethersulfone was performed by adding sulfonic acid group to the PES using triethyl phosphate and sulfuric acid as the sulfonating agent at 75˚C for 2 hours. Phase separation technique was applied to make a ultrafiltration membranes from a mixture of sulfonated poly ethersulfone and polyethersulfone as polymers, polyethylene glycol 400 and polyethylene glycol 6000 as additives and 1-Methyl-2-pyrrolidinone as solvent in different temperatures of non-solvent bath to form membranes of varying pore size. Sulfonaton identification was performed using Fourier transform infrared spectroscopy. The protein separation as function of pH value was studied with egg white to separate lysozyme using UF. Analysis by UV spectroscopy exhibited high selectivity in separation of the lysozyme because of membrane charge density. The membranes shows less protein solution fluxes in dead end ultrafiltration compared to cross flow ultrafiltration due to concentration polarization. The highest separation of lysozyme obtained for membrane SP-27 (SPES+PEG400, non solvent bath temperature 25˚C) because of its suitable porosity, hydrophilicity and appearance of clear lysozyme band in sodium dodecyl sulfate polyacrylamide gel electrophoresis without complex molecular weight protein bands with egg white protein solution flux 5.64 Lt/m2.hr in cross flow ultrafiltration at 15 psi pressure. Keywords - Lysozyme; S-PES Membrane; Ultrafiltration, Flux, UV spectroscopy, Electrophoresis.