Anti-Aging Properties of Traditional Asian Herbal Plants: An Experimental Study on Centella Asiatica L. and Acalypha Indica L. Plants Extract
Introduction: In 2050, it is estimated that the world will have 1.5 billion people aged 65 or above. This trend will pose a challenge because of the association between increasing age and incidence of chronic diseases. This alarming statistics calls for a new kind of intervention to combat the disease. While good curative treatments are paramount, high-quality treatments are still not adequately available in many countries, such as Indonesia. Moreover, curative measures are not quite effective in treating chronic diseases. Therefore, we propose the use of antioxidants to prevent chronic diseases. However, the use of synthetic antioxidants chronically may incur side effects and is expensive. As an agricultural power, Indonesia is home to many different types of plants. Two of which are Centella asiatica L. (CA) and Acalypha indica L. (AI) These plants have been used empirically by many societies and might be potential natural alternatives of antioxidants to combat the rise of aging and chronic diseases.
Method: Research was conducted experimentally on aged Sprague-Dawley mice using five groups, notably negative control using placebo, positive control using conventional antioxidant vitamin E, test group using 250 mg/kg BW AI extract, another test group using 300 mg/kg BW CA extract, and comparison control using young mice. After 28 days, the mice were terminated. The kidneys of the mice were tested for TNF-alpha concentration using ELISA kit. Subsequent data were analysed using Saphiro-Wilk test and one-way ANOVA.
Results & Discussion: Based on the experiment results, both administration of CA and AI yields no statistically significant decrease in TNF-alpha levels of aged mice kidney in comparison to placebo and positive controls (p=0.617 and p=0.535, respectively). However, a subtle decrease in TNF-alpha levels is still notable in both case. Our foundings suggest a potential anti-inflammatory effect of both CA and AI had the treatment been done longer nor with bigger dose of administration, this correspondents to studies showing potent anti-aging effects of CA and AI having administering time of minimum 2 months. Conclusion: All in all, further research and exploration needs to be made on CA and AI, including more variations of bigger dose and longer time of administration.
Keywords - Antiaging, TNF-Alpha, Kidney, Centella Asiatica, Acalypha Indica