Paper Title
Analysis Dihydrolipoamide Dehydrogenase (DLD) and Kidney Injury Molecule-1 (Kim-1) MRNA Exspression in Kidney Model of Initiation Phaseischemia - Reperfusion Injury(I-RI)

Abstract
Dihydrolipoamide dehydrogenase (dld) is a complex enzyme subunit of three important enzymes in mitochondria. Kidney injury molecule-1 (KIM-1) is a type I transmembrane molecule in renal proximal tubular epithelial cells. The basic relationship between I-RI is mitochondrial dysfunction caused by ROS burst. This study uses a laboratory experimental method in Wistar rats (RattusNorvegicus). Renal ischemia is performed by clamp the renal a / v using a bulldog clamp. The monitoring samples were 30 and were grouped into 5 groups namely healthy controls (K), Ischemia 30 '(I), reperfusion 30' (IR 30 '). 2 hour reperfusion (2J IR), 24 hour reperfusion (24J IR).Dld and KIM-1 mRNA expressions can use RT-PCR. The results of the measurement of gene expression by normalizing the reference gene using the Livak method are 2-∆∆CT, comparing the values of KIM1 and DLD expression in several treatment groups (Klp K, I, IR 30 ', IR 2J, IR 24J). Conclusion: KIM-1 expression was higher (1.4 times) in the Ischemia group 30 '(I) compared to the control group. DLD Expression is higher (5 times) compared to control. Conversely, in ischial reperfusion, mLNAdld expression and KIM-1 are lower (down expression) than controls which are interpreted as unexpressed. . Keywords - Dihydrolipoamide dehydrogenase (dld), Kidney injury molecule-1 (KIM-1), ischemia, ischemiareperfusion.