Effect of Medium Ph and Sucrose Concentrations on Adventitious Roots Induction of Labisia Pumila
Adventitious roots are roots that arise from parts of the plant not originating from the embryonic root; such as roots originating from stems or leaves. Adventitious roots are a potential source for the production of valuable plant secondary metabolites on a commercial scale. A study was undertaken to investigate the effects of medium pH and sucrose concentrations on adventitious roots induction from leaf explants of Labisia pumila. Full strength of Murashige and Skoog (MS) medium supplemented with 5 mg/L of indole-3-butyric acid (IBA) were used in this study. Tukey�s analysis showed that there was a significant difference between the parameter studied with the percentage of rooting, number of roots per explants and dry weight of roots. pH 6.0 is the optimum pH value for the induction of L. pumila roots compared to pH 4.0, 5.0, 5.8 and 7.0. The highest rooting efficiency was evidenced by the highest value recorded in rooting percentage (89.3 � 9.5 %), (13.4 � 0.99) roots per explants and (0.0364 � 0.005g) dry weight of roots. It showed that 5% of sucrose was the optimum concentration for L. pumila as compared to 0, 1, 2, 3, 4, 6, and 7 % that produced lower rooting efficiency. The highest value was recorded at 5 % of sucrose with a rooting percentage (72 � 13.2 %), (10 � 4.45) roots per explants and (0.044 � 0.014 g) dry weight of roots. No adventitious roots formed in MS media without sucrose.
Index Terms- Adventitious root, carbon source, Labisia pumila, medium pH, sucrose