Paper Title
Glutathione S-Transferase M1 and T1 Genes Polymorphism Association with Antituberculosis Drug-Induced Hepatotoxicity in Georgian Population

Abstract
The glutathione S-transferase (GST) enzyme system constitutes a family of multifunctional enzymes which play an important role in biotransformation and detoxification of many different xenobiotic. Human cytosolic GSTs are polymorphic, and have ethnic-dependent polymorphism frequencies, and have been associated with several types of diseases. In the double-null variant of GSTM1 and GSTT1(GSTT1(-) and GSTM1(-)) are respectively associated with a higher risk of different forms of liver injury, cancer, cardiovascular diseases etc[1-3]. Comparisons between GST null genotypefrequencies in the worldwide populations show different patterns in Asian, African, and European populations. Some detailed studies of GST variants in various geographic regions can increase the knowledge about relationship between the ethnicity and the prevalence of certain diseases[4]. Thus, it becomes necessary to consider the genotypic differences for reducing the risk of anti-tuberculosis drug-induced Liver injury[5]. The aim of our research was to determine the polymorphism of GSTM1 and GSTT1 genes in Georgian population – in healthy individuals and in patients with lung tuberculosis. In the studies was used the peripheral blood samples obtained from individuals randomly selected groups and from patients with lung tuberculosis. The GSTM1 and GSTT1 genotypes was investigated using an Ese-Quant tube scanner-by SmartAmp Method[6]. The study of GST genes polymorphism in Georgian population revealed that only 1% of healthy individualshave double null genotype by GST genes (GSTT1(-) and GSTM1(-)). As for patients with lung tuberculosis, there was revealedthat GSTT1(-)GSTM1(-) genotype was in 14% of investigated patients. All patient with double null genotype hasviolation of liver functional indicators. Based on our results we can conclude, that antituberculosis drug induced hepatotoxicity in Georgian Population is associated with the existence of a double null genotype of GST genes. The results will play the most important role in personalized medicine, in the appointment and management of drugs and for prevention of adverse drug reactions in patients of Georgian population. Keywords - GST Genes; Lung Tuberculosis; SmartAmp Method.