Paper Title
Interaction of Latent Epstein-Barr Virus and Cytomegalovirus Reactivation with Pain Levels between Two Different Age Groups

Abstract
Inflammageing,immunosenescence and aging altogether serve as initiator for pain level by increasing the opportunity of herpesviruses reactivation by (EBV and CMV) after latent period, because of impaired immunity.So, the current study assumed to study the potential interaction between these viruses and pain levels, which had been evaluated by interleukins (ILs-6, 7) and CRP estimation in two different age groups. Serum samples of two age groups (100 participants) as young ages (ranged: 20 – 35) and old ages (≥ 50) years, were examined for antibody titers of (EBV VCA and CMV IgGs), interleukins (ILs-6, 7) and CRP levels by the method of Enzyme Linked Immunosorbent Assay (ELISA). Besides, pain (SF-36 pain subscale) was assessed between these groups. Interestingly, the old ages (≥ 50 years) were with a strong significant positive association (p<0.001; r = 0.689) with higher pain levels (65.402±13.566) and high titer of serum EBV VCA-IgG (2.1816±0.558), followed by week positive correlation with CMV-IgG as a latent herpesviruses reactivation. Furthermore, multiple linear regression test suggested that higher pain levels were associated with variation in age groups (p<0.001), EBV, CMV and IL-6 (p<0.05) among individuals with higher pain but not CRP or IL-7. it was concluded, higher pain level was estimated among the old ages of participants and it was consequently related with EBV and CMV reactivation and up-regulation of IL-6. Keywords - CRP, CMV, EBV, IL-6, IL-7, Pain.