Paper Title
Isolation and in Silicocharacterization of UGE Gene in Klebsiella Pneumonia Isolated from Indoor Dust

Abstract
Dust includes many micro-organisms such as bacteria named as airborne bacteria. Most types of these bacteria are harmful to humans and causing severe problems. Klebsiellapneumoniae consider a serious nosocomial pathogen that cause pneumonia, UTIs, wound and liver abscesses. One hundred fifty (150) samples isolated randomly from different indoor and outdoor areas during the period November 2014- February 2015. In the indoor isolates bacteriumKlebsiellapneumoniae was the commonest pathogen (36.70%) followed by Staphylococcus aureus (22.78%), Staphylococcus epidermidis (12.65%), Bacillus spp. (10.12%), Pseudomonas aeruginosa (5.06%), Enterobacter cloacae (5.06%), Streptococcus spp.(2.53%), Citrobacter spp.(1.26%) and Pantoea spp.(1.26%) respectively. While the pathogen Staphylococcus aureuscomprises the majority of outdoor bacteria as isolated in (41.66%) followed by Bacillus spp.(22.22%), Klebsiellapneumoniae(19.44%), Staphylococcus epidermidis (5.55%), Pseudomonas aeruginosa (5.55%), Aeromonas spp. (2.77%) and Chromobacteriumviolaceum (2.77%) respectively. All K. pneumoniaeisolates detected and confirmed by conventional PCR using Ugegene. The positive isolates were sequenced and search for homology. The multiple sequence alignment showed that our bacterial gene have differences in NAD-dependent epimerase and NAD(p)- binding domain sequence at different sites.These differences in the gene sequence may indicates special characterizations for theisolated strain which may give new approach for developing higher sensitive K. pneumoniae strains. Keywords - In silico, Klebsiella pneumonia, Dust, Air borne, Phylogenetic